发明名称 |
VERFAHREN ZUR BESTIMMUNG VON POLYPEPTIDEN |
摘要 |
Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more epsilon-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint. |
申请公布号 |
DE60215498(T2) |
申请公布日期 |
2007.04.26 |
申请号 |
DE2002615498T |
申请日期 |
2002.06.07 |
申请人 |
ELECTROPHORETICS LTD. |
发明人 |
HAMON, CHRISTIAN;THOMPSON, ANDREW;NEUMANN, THOMAS;JOHNSTONE, ROBERT;MOHAMMED, ABDUL KARIM ABED |
分类号 |
G01N27/62;G01N33/68;C07K1/12;G01N30/88;G01N33/483 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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