发明名称 |
HUMAN IMMUNODEFICIENCY VIRUS ENTRY INHIBITOR ASSAY |
摘要 |
The present invention is a method for evaluating the ability of a compound to prevent the Human Immunodeficiency Virus ("HIV") from entering into a T cell. The method involves fusing a first cell line that mimics HIV viral particles by expressing a gp120-gp41 complex on the cell surface and that contains a Tat protein in the cytoplasm with a second cell line that mimics T cells by expressing CD4 and its co-receptors on the cell surface and that contains a Tat-inducible reporter gene expression cassette in the nucleus. The cell lines are fused alone or in the presence of a potential HIV entry inhibitor and the amount of beta-galactosidase produced by the fused cells is determined. Compounds that reduce the amount of 0-galactosidase produced by the fused cells have interfered with the interaction of the gp120-gp41 complex and CD4 and its co-receptors and are therefore deemed be HIV entry inhibitors.
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申请公布号 |
US2007092939(A1) |
申请公布日期 |
2007.04.26 |
申请号 |
US20040580703 |
申请日期 |
2004.11.24 |
申请人 |
ZOU LINGLONG |
发明人 |
ZOU LINGLONG |
分类号 |
C12Q1/68;C07K;C12P21/06;C12Q1/70;G01N33/50;G01N33/53;G01N33/536;G01N33/569;G01N33/574 |
主分类号 |
C12Q1/68 |
代理机构 |
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主权项 |
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地址 |
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