发明名称 |
PURIFICATION, CHARACTERIZATION AND RECONSTITUTION OF A UBIQUITIN E3 LIGASE |
摘要 |
The present invention provides a native or reconstituted complex comprising Bmi1 and/or Ring1 and/or Ring2, wherein the complex has ubiquitin E3 ligase activity. Optionally, the complex further comprises HPH2 and/or HPC3. Also disclosed are methods of producing the reconstituted complex, methods of identifying compounds that modulate the ubiquitin E3 ligase activity of the native or reconstituted complex, and methods of identifying candidate compounds for treating cancer. Kits for determining modulation of protein ubiquitination and/or for ubiquitinating a target substrate are further provided. |
申请公布号 |
WO2006130720(A3) |
申请公布日期 |
2007.04.26 |
申请号 |
WO2006US21159 |
申请日期 |
2006.06.01 |
申请人 |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL;ZHANG, YI;CAO, RU;WANG, HENGBIN |
发明人 |
ZHANG, YI;CAO, RU;WANG, HENGBIN |
分类号 |
G01N33/48;C12M1/34 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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