| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a new method for purifying recombinant proteins directly usable in the diagnosis and vaccine of human hepatitis C virus. <P>SOLUTION: A method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2 is provided, being characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein, a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. A composition isolated by such a method, the diagnostic and therapeutic application of these compositions, and furthermore, the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment, are also provided, respectively. <P>COPYRIGHT: (C)2007,JPO&INPIT |
