| 摘要 |
Nucleic acids, including DNA constructs and RNA transcripts, capable of indu cing coordinate expression of two to three cistrons upon direct introduction into animal tissues, are presented. Bi- or tri-ci stronic polynucleotides of this invention include those encoding and co-expressing HIV gene products, genes encoding antigens unrelated to HI V, and immunostimulatory gene products, including but no t limited to GM-CSF, interleukins, interferon and members of the B7 family of proteins which act as T-cell costimulatory elements. T he methods and polynucleotides of this invention are generally applicable to co -ordinate expression in vivo of any two or more genes in a single cell.
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