Methods and compositions for using oils for analysis and detection of molecules

摘要

Oil compositions and methods for use in droplet formation or in spacing of droplets are disclosed. The oil compositions may include a combination, such as a silicone oil and a fluorine-containing compound. The droplets can contain analytes (e.g., nucleic acids) and may be used for molecular reactions (e.g., digital PCR) and detection.

主权项

1. A composition comprising:
aqueous droplets; and a homogeneous oil mixture forming a continuous phase that encapsulates each of the aqueous droplets, the oil mixture including (a) a silicone oil and (b) a fluorinated oil; wherein the concentration of said silicone oil in the oil mixture is at least about 50% by weight, and wherein the aqueous droplets have a neutral buoyancy in the oil mixture.