| 摘要 |
The present disclosure is related to a thermocycler device of the type that allow to amplify DNA through Polymerase Chain Reaction in microfluidic devices, comprising a low power resistor which is used as a heater and a fan that is implemented as heatsink where the temperature cycles are achieved programming a Proportional-Integral-Derivative control in serial communication with a microcontroller from a personal computer, in such a way that, through the pulse-width modulation it is possible to regulate the voltage drop in the resistor which acts as a heater as well as in the fan or heatsink to obtain the desired temperature, where in addition, to monitor the temperature in real time, a K-type thermocouple is used; and which is conditioned to an analog input of the same microcontroller. |
