MSCA1 NUCLEOTIDE SEQUENCES IMPACTING PLANT MALE FERTILITY AND METHOD OF USING SAME

摘要

Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.

主权项

1. A method of maintaining a homozygous recessive condition of a male sterile plant, the method comprising:
(a) providing a first plant comprising homozygous recessive alleles of a Msca1 gene and is male sterile; (b) providing a second plant comprising homozygous recessive alleles of the Msca1 gene and a construct in the hemizygous condition, the construct comprising:
(i) a first nucleotide sequence comprising the Msca1 nucleotide sequence, that when expressed in the first plant would restore male fertility;(ii) a second nucleotide sequence that when expressed inhibits the function or formation of viable male gametes in the second plant, such that viable male gametes are produced in the second plant containing the recessive alleles of Msca1 and that do not contain the construct; and (c) fertilizing the first plant with the male gametes of the second plant to produce progeny which maintain the homozygous recessive condition of the first plant.