NEW MEDIUM FOR HIGH PERFORMANCE MAMMALIAN FED-BATCH CULTURES

摘要

The present invention relates to new serum- and protein-free culture media. These media are high performance culture media, which notably improve mammalian fed-batch cultures. The present invention also relates to methods for preparing and/or designing the medium, and methods of use thereof.

主权项

1. A cell culture medium comprising NaH2PO4, L-Leucine, L-Lysine, L-Methionine, L-Glutamic acid, L-phenylalanine, L-proline, L-threonine, L-tryptophan, L-Valine, magnesium sulfate, calcium chloride, myo-inositol, sodium pyruvate, D-Biotin, choline chloride, L-Aspargine, folic acid, niacinamide (B3), D-pantothenic acid×½Ca, L-Serine, potassium chloride, pyridoxine, L-Aspartic acid, riboflavin, thiamine, ferric ammonium citrate, vitamin B12, hypoxanthine, thymidine, putrescine, ethanolamine, zinc sulfate, cupric sulfate, pluronic, L-tyrosine, sodium selenite, L-arginine, L-Cysteine, L-Histidine and L-Isoleucine, wherein the medium is serum and protein free.