| 摘要 |
Disclosed herein are fully impaired consensus Kozak sequences which are most typically used with dominant selectable markers of transcriptional cassettes which are a part of an expression vecto r. These vectors are most typically utilized in the expression of proteins in mammalian expression systems. As defined, disclose d and claimed herein, a "fully impaired consensus Kozak" comprises sequence (I), where: "x" is a nucleotide selected from the group consisting of adenine (A), guanine (G), cytosine (C) or thymine (T)/uracil (U); "Py" is a pyrimidine nucleotide, ie C or TU; "ATG" is a codon encoding for the amino acid methionine, the so-called "start" codon; and -3 and + 1 are directional reference points vis-a-vis ATG, ie -3 is meant to indicate three nucleotides upstream of ATG and + 1 is meant to indicate one nucleotid e downstream of ATG. Dominant selectable markers further comprising artificial intronic insertion regions are further disclosed.
|
