A DETOXIFIZYME HAVING ACTIVITY OF TRANSFORMING AFLATOXIN AND THE GENE ENCODES THEREOF.

摘要

The invention relatives to a type of detoxifizyme with the activity of transforming aflatoxin and the gene encodes thereof. The inventors firstly isolate and purifya novel protein, named aflatoxin-detoxifizyme(ADTZ), which processes the transforming activity. The primes specify to the ADTZ gene are obtained through purification and sequencing. The gene encoding ADTZ is cloned from the totalRNA of Armillariella tabescens, and the recombinant protein is expressed and purified through various expression systems by genetic engineering methods.The said detoxifizyme has bioactivity of transforming AFB1, reducing the mutagenic effect of AFB1, which provides a excellent base for feed production, food industry and development of anti-tumour drugs.