METHOD OF CELL CULTURES AND DEVICE FOR IMPLEMENTING IT

摘要

Cell culture device (1) comprising a culture vessel (2) provided with a cover (19) in which there is a first zone (3) for transferring culture medium essentially free from cells internal to a second cell culture zone (5), a third (4) and a fourth zone (6) for transferring culture medium essentially free from cells. The third zone (4) is internal to the second zone (5) and external to the first zone (4) and the fourth zone (6) being a zone external to said second zone (5). All the zones are in medium communication. The device also comprises culture medium circulation means, allowing a circulation of the culture medium through the culture zone (5) from bottom to top.