| 摘要 |
The present invention provides a method of producing a cell population enriched for osteoclasts in which bone marrow mononuclear cells (BMNC) are cultured in a medium supplemented with growth factors, cytokines, or a combination thereof for at least 7 days to obtain colony-forming units of granulocytes and macrophages (CFU-GM); CFU-GM are isolated from culture by centrifugation; and isolated CFU-GM are cultured for a period of time and under conditions sufficient for CFU-GM to differentiate to osteoclasts. The invention further provides a cell population produced by the methods described.
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