<p>The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.</p>
申请公布号
WO2007041713(A1)
申请公布日期
2007.04.12
申请号
WO2006US39139
申请日期
2006.10.04
申请人
ZYMOGENETICS, INC.;ZAMOST, BRUCE, L.;LEE, GEOFFREY, F.;DEDINSKY, ROBERT, M.
发明人
ZAMOST, BRUCE, L.;LEE, GEOFFREY, F.;DEDINSKY, ROBERT, M.