| 摘要 |
A monoclonal antibody which selectively binds to all forms of thymidine kinase, including TK2, active and inactive forms of TK1, and monomeric and multimeric forms of TK1, is described. Also described ate monoclonal antibodies which selectively bind only to active or tetrameric TK1 and to TK2, respectively. A panel of antibodies including two or more of the above-described antibodies is useful to correlate levels of TK1 separately from total TK or TK2, according to a meth od described in the application. Methods for producing the above-noted monoclonal antibodies are also described, including a method for obtaining sufficient quantities of pure active/multimeric TK1 for use as an antigen. The anti-TK antibodies in the panel are useful individually and in various combinations to test tissue samples of human tumor patients, to predict from a primary tumor the likelihood of tumo r recurrence, and to monitor treatment effectiveness. The antibodies are also useful for diagnosing, staging, and monitoring treatment efficacy in tumors of other kinds.
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