发明名称 |
Isolation and characterization of a cDNA clone encoding one IgE-binding fragment of Penicillium brevicompactum |
摘要 |
Pen b 26 was isolated as an allergen of Penicillium brevicompactum, which is known to be one of the major source of indoor fungal allergies. Pen b 26 was isolated and cloned into Escherichia coli as an N-terminus his-tagged fusion protein, which had a calculated molecular weight of 14.9 kDa while the native Pen b 26 was 11 kDa. The over-expressed fusion protein migrated at about 20 kDa on SDS-PAGE although its analysis by mass spectroscopy confirmed its calculated size. The IgE antibodies in the sera of >25% Penicillium-allergic individuals showed positive reaction to the purified fusion protein. Pen b 26 was also identified as a 60S ribosomal P1 phospho-protein. This invention includes a cDNA sequence from which the recombinant allergen was produced.
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申请公布号 |
US2007077255(A1) |
申请公布日期 |
2007.04.05 |
申请号 |
US20060528679 |
申请日期 |
2006.09.28 |
申请人 |
SEVINC M S;VIJAY HARI M |
发明人 |
SEVINC M. S.;VIJAY HARI M. |
分类号 |
A61K39/00;C07H21/04;C07K14/385 |
主分类号 |
A61K39/00 |
代理机构 |
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地址 |
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