| 摘要 |
Homologous viral internal controls have been developed for use in RT-PCR assays of a number for viruses. Two of these internal controls, such as for HAV and Norwalk virus, are cloned on a single plasmid, thereby making them useful for the multiplex detection of these viruses in a single reaction. These controls avoid the problem of inhibitors for RT-PCR that may be present in sample water assayed. These internal controls can also potentially be used in the detection of viruses in other matrices such as blood or cerebrospinal fluids or in other clinical applications. The development of two internal controls on a single plasmid enables multiplexing of multiple virus detection, thereby saving time, cost and labor.
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