| 摘要 |
<p>A method of detecting a ligand comprises providing a receptor for a predetermined ligand. The receptor comprises a conductive material. The receptor is contacted with a sample in the presence of a non-conductive medium. When the ligand is present in the sample, the receptor and the ligand form a receptor-ligand complex that is capable of forming a conductive aggregate. A second receptor is then provided. The second receptor is a receptor for the conductive aggregate, and the second receptor is bound to a pair of electrodes. The electrodes are electrically separated from each other in the absence of the conductive aggregate, and are electrically communicating in the presence of conductive aggregates. The method also includes providing a power source in electrical communication with the electrodes, such that an electric circuit is selectively completed in the presence of conductive aggregates, signaling the detection and capture of the ligand. A cassette for performing this method is also provided.</p> |
