Verfahren zur Herstellung einer genetisch-verändereten Pflanze

摘要

The present invention is related to a method for obtaining a genetically modified plant comprising the steps of : (i) transforming protoplasts, preferably derived from a conversion of stomatal cells, by introducing an hereditary material into the protoplasts of said plant in order to obtain transformants containing at least one copy of the hereditary material per protoplast, (ii) selecting a stable transformant and (iii) regenerating plant cells or a whole plant from said transformant, characterised in that the hereditary material is a cassette nucleotide sequence to be inserted in the genome of said plant and being free or substantially free from prokaryotic or plasmidic nucleotide sequences.