PROTEIN COMBINATION CHARACTERIZED IN FLUORESCENCE RESONANCE ENERGY TRANSFER, AND THE USE THEREOF

摘要

The invention relates to a kind of protein combination characterized in fluorescence resonance energy transfer, which consists of the protein of variable region in heavy chain and the protein of variable region light chain from the same antibody, and optionally, specific antigen recognized by the antibody, wherein either of the protein of variable region in heavy chain and the protein of variable region light chain or the optional specific antigen recognized by the antibody has fluorescence substance (chemiluminescence substance) as energy donor, or has fluorescence quencher as energy acceptor. The invention also relates to a method of detecting the target antigen in a sample by using the protein combination, and the kit containing the target antigen in the sample detected by the protein combination.