发明名称 |
A method for isolating RNA |
摘要 |
<p>A method for isolating RNA from RNA containing samples wherein the RNA containing sample is treated with at least one DNase and/or another enzyme like proteases or collagenases for purification of RNA in presence of a complex of ribonucleosides and the oxovanadyl ion which is capable of inhibiting RNases present in the RNA containing samples and removing the complex of ribonucleosides and the oxovanadyl ion prior to down-stream processing of the RNA of the RNA containing sample by contacting the sample of the forgoing step with a chelating agent immobilized to a support, the chelating agent having sufficient affinity to the complex of ribonucleosides and the oxovanadyl ion so that it binds to the chemical entity and the RNA containing sample is freed from the inhibitor.</p> |
申请公布号 |
EP1767631(A1) |
申请公布日期 |
2007.03.28 |
申请号 |
EP20060121200 |
申请日期 |
2006.09.25 |
申请人 |
NEXTTEC GMBH |
发明人 |
FRITZEMEIER, NINA;BREM, GOTTFRIED;LEISER, ROBERT-MATTHIAS;BALAYAN, HAMLET |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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