| 摘要 |
A method for preparing a skin functional peptide combined with a fatty acid is provided to easily produce a peptide combined with an oleic acid in a nanosome form, thereby increasing the percutaneous absorption rate and effectively removing skin wrinkle within short period of time in a wide range. The method comprises the steps of: (a) reacting oleic acid with diisopropylcarbodiimide in the presence of dimethyl formamide; (b) after reacting peptide with dimethyl formamide, adding it to the resultant product obtained from the step(a); (c) after removing the dimethyl formamide using a centrifugal separator after the completion of the reaction, putting methanol therein to remove foreign materials and the remaining dimethyl formamide therefrom; (d) removing the methanol from the peptide using a centrifugal separator and nitrogen gas; (e) reacting the methanol removed peptide with trifluoroacetic acid(TFA) cleavage solution; (f) after filtering the reaction product obtained from the step(e) with a filter, dropping the solution filtered through the filter in diethyl ether to generate precipitate; (g) removing the diethyl ether and TFA cleavage solution from the precipitate-formed peptide using a centrifugal separator; (h) after putting diethyl ether therein, removing supernatant therefrom using a centrifugal separator; and (i) removing the diethyl ether from the peptide using nitrogen gas. After the step(i), a step of dispersing the product obtained from the step(i) in oleic acid and a step of passing the dispersion through a micro-fluidizer for 5-10 times to obtain a nanosome shaped peptide is further performed.
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