| 摘要 |
FIELD: medicine, molecular-biological researches, oncodiagnostics. ^ SUBSTANCE: the declared method and multi-purpose diagnostic kit (DK) are indicated for carrying out medico-genetic analysis of simultaneously several mutations (polymorphisms) of different genes, those associated with some frequent multifactorial diseases, among them. Due to applying standard component of the mixture and standard oligoprimers, optimizing the conditions for performing polymerase chain reaction (PCR) and analyzing polymorphism of restriction fragments length (PRFL) the rate of such an analysis has been shortened up to 2-3 d. The innovation simplifies and cheapens the analysis in question and makes it useful for carrying out molecular investigations both in specialized centers and in multi-purpose laboratories. ^ EFFECT: higher accuracy and efficiency of detection. ^ 2 cl, 2 tbl |
