WEST NILE VIRUS VACCINE

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a West Nile Virus (WNV) vaccine and to obtain an attenuated WNV vaccine produced by the method. SOLUTION: The method for producing a WNV vaccine comprises culturing an established cell line (Vero cells) originating in the kidney of a WNV-inoculated African green monkey at a high density, attenuating the WNV-infected cells or a liquid culture medium thereof thus obtained and then highly purifying WNV by a sucrose density gradient centrifugation method. The WNV is obtained by the method. The attenuated WNV vaccine comprises the WNV or its antigenic component as a main component and is substantially free from any nucleic acids or proteins originating in a host. COPYRIGHT: (C)2007,JPO&INPIT