NOVEL LOOP SEQUENCE EFFECTIVE FOR EXPRESSION OF shRNA

摘要

Disclosed is a loop sequence having high gene-suppressive effect by utilizing shRNA. Short RNA expressed in a human cell is purified and screened by a comprehensive gene expression analysis technique (MPSS) using microbeads, and the sequence of the RNA is analysed. Based on the sequence information given by the analysis, a database analysis is made. Among the sequences showing 100% matching to the human genome, a sequence which does not hit known miRNA, rRNA or tRNA is extracted. Based on the prediction of secondary sequence, a loop sequence is obtained.