| 摘要 |
PROBLEM TO BE SOLVED: To provide an inexpensive and highly sensitive method for determining DNA sequence without using an expensive reagent in a stepwise complementary chain-synthesizing reaction. SOLUTION: dATP in an amount not exceeding 50 times of the amount of a template DNA is added to a reactor containing the template DNA; stepwise complementary chain synthesis is carried out; background emission intensity originated from dATP in which dATP becomes a substrate of luciferase and emits light is deducted from the measured luminescence; and luminescence resulting from complementary chain synthesis caused by ATP converted and produced from pyrophosphoric acid produced in complementary chain synthesis is obtained. Thereby, determination of base sequence of the template nucleic acid is carried out. COPYRIGHT: (C)2007,JPO&INPIT
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