发明名称 |
LDL receptor signaling assays |
摘要 |
The invention provides methods and compositions for modeling and detecting LDL receptor transmembrane signaling by detecting proteolysis of an LDL receptor transmembrane domain. The method comprises the steps of: a) providing a sample comprising a cell membrane comprising (i) a polypeptide comprising an LDL receptor transmembrane domain fused to a C-terminal tail, and (ii) a protease which specifically cleaves the domain and thereby releases the tail from the membrane; b) incubating the sample under conditions wherein the protease cleaves the domain and thereby releases the tail from the membrane; and c) detecting a resultant released tail.
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申请公布号 |
US7192714(B2) |
申请公布日期 |
2007.03.20 |
申请号 |
US20010977155 |
申请日期 |
2001.10.12 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
HERZ JOACHIM;MAY PETRA |
分类号 |
G01N33/53;C12Q1/37;G01N33/92 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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