发明名称 |
Biosynthesis of amorpha-4,11-diene |
摘要 |
Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.
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申请公布号 |
US7192751(B2) |
申请公布日期 |
2007.03.20 |
申请号 |
US20030411066 |
申请日期 |
2003.04.09 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
KEASLING JAY;MARTIN VINCENT;PITERA DOUGLAS;WITHERS, III SYDNOR T.;NEWMAN JACK |
分类号 |
C12P7/42;C07H21/04;C12N1/20;C12N1/21;C12N9/00;C12N9/10;C12N15/00;C12N15/74;C12P9/00 |
主分类号 |
C12P7/42 |
代理机构 |
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代理人 |
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地址 |
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