发明名称 |
Isolated DNA molecules encoding humanized calcitonin gene-related peptide receptor, related non-human transgenic animals and assay methods |
摘要 |
Disclosed herein are isolated nucleic acid molecules encoding a humanized version of a calcitonin gene-related peptide (CGRP) receptor, which comprises the G-protein coupled receptor calcitonin-receptor-like receptor (CRLR) and the receptor-activity-modifying protein 1 (RAMP1). The humanized CGRP receptors of the present invention attain pharmacological profiles similar to the wild type human receptor via modifications to the respective mammalian RAMP1 nucleotide sequence, specifically at amino acid 74. Also described are related recombinant vectors, recombinant hosts and associated methods for generating such humanized CGRP receptors. Also presented are non-human transgenic animals which express humanized RAMP1. Such animals have been engineered to provide for a CGRP pharmacological profile similar to human CGRP.
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申请公布号 |
US7193070(B2) |
申请公布日期 |
2007.03.20 |
申请号 |
US20040490594 |
申请日期 |
2004.03.23 |
申请人 |
MERCK & CO., INC. |
发明人 |
KANE STEFANIE A.;SALVATORE CHRISTOPHER A.;MALLEE JOHN J.;KOBLAN KENNETH S.;OLIVER KEVIN R. |
分类号 |
A01K67/027;C07K1/00;C07H21/02;C07H21/04;C07K14/00;C07K14/47;C07K14/705;C07K17/00;C07K19/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/64;C12N15/00;C12N15/09;C12N15/85;G01N33/15;G01N33/50 |
主分类号 |
A01K67/027 |
代理机构 |
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主权项 |
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地址 |
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