发明名称 |
IMPROVED NUCLEIC ACID AMPLIFICATION PROCEDURE |
摘要 |
The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA- containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products. |
申请公布号 |
WO2007030759(A2) |
申请公布日期 |
2007.03.15 |
申请号 |
WO2006US35154 |
申请日期 |
2006.09.07 |
申请人 |
NUGEN TECHNOLOGIES, INC.;KURN, NURITH;WANG, SHENGLONG |
发明人 |
KURN, NURITH;WANG, SHENGLONG |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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