METHOD FOR INCREASING THE CONTENT OF POLYUNSATURATED LONG-CHAINED FATTY ACIDS IN TRANSGENIC ORGANISMS

摘要

The invention relates to a method for increasing the content of polyunsaturated long-chained fatty acids in an organism, by introducing nucleic acid coding for polypeptides and/or proteins having phopholipase, ketoacyl-CoA-reductase and/or dehydratase activity into the organisms. Advantageously, said enzymes stem from ostreococcus or thraustochytrium. The invention also relates to nucleic acid sequences, nucleic acid structures, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid structures, and transgenic organisms containing the above-mentioned nucleic acid sequences, nucleic acid structures and/or vectors. Advantageously, said above-mentioned nucleic acid frequencies, nucleic acid structures and vectors can optionally be expressed in the organism along with additional nucleic acid sequences coding for the polypeptides and/or proteins of fatty acids or lipid metabolism biosynthesis. Another advantage of the nucleic acid sequences of the fatty acids or lipid metabolism is that they are coded for ?-9 elongase, ?-8-desaturase, a ?-5-desaturase, ?-4-desaturase, ?-12-desaturase, ?-5-elongase and/or ?-6-elongase activity. Said desaturases and elongases stem from organisms such as thalassiosira, euglena, isochrysis, physcomitrella, thraustochytrium, borago, phytophthora, crypthecodinium, oncorhynchus, primula, xenopus, ciona, arabidopsis, mortierella, caenorhabditis, phaeodactylum, ceratodon or ostreococcus. The invention further relates to oils, lipids, and/or fatty acids that are produced according the inventive method, and to the use thereof, in addition to the inventive unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and to the use thereof.