HIGHLY SENSITIVE ENZYME IMMUNOASSAY SYSTEM FOR QUANTIFICATION OF SURFACE ANTIGEN (HBsAg) OF HEPATITIS B VIRUS (DIA-HBsAg-sensitive)

摘要

The highly sensitive enzyme immunoassay system for the quantification of the surface antigen (HBsAg) of hepatitis B virus (DIA-HBsAg-sensitive) has been developed. The immune sorbent represents the monoclonals against the surface antigen of hepatitis B virus. The recombinant HBsAg at the specified concentration is used the positive control. The conjugate contains biotinilated antibodies against HBsAg and streptavidin coupled with peroxydase. To amplify the signal, biotintiramine and peroxidase-labeled steptavidin are added to the system.