METHOD FOR IN SITU HYBRIDIZATION ANALYSIS

摘要

The present invention is a method for in situ hybridization analysis of the temporal and/or spatial expression of one or more ribonucleic acid target sequences in a tissue sample. The disclosed method can be adapted for high- throughput analysis of a plurality of target sequences and a plurality of tissue samples and the method is highly sensitive based upon the combination of a radiolabeled ribonucleic acid probe and silver emulsif ication of the tissue sample to detect the temporal and/or spatial expression of the target sequence.