发明名称 |
Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry |
摘要 |
The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.
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申请公布号 |
US2007059776(A1) |
申请公布日期 |
2007.03.15 |
申请号 |
US20060499898 |
申请日期 |
2006.08.03 |
申请人 |
CIPHERGEN BIOSYSYSTEMS, INC. |
发明人 |
BOSCHETTI EGISTO;BRADBURY LISA;DAVIES HUW;LOMAS LEE O.;PHAM THANG T.;THULASIRAMAN VANITHA;YIP TAI-TUNG |
分类号 |
G01N27/62;G01N33/567;C07K1/16;C07K1/18;C07K1/20;C07K1/22;C07K14/47;C12P21/02;G01N27/64;G01N30/72;G01N30/84;G01N30/88;G01N33/53;G01N33/68 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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