Method for the activation of a nucleic acid for performing a polymerase reaction

摘要

Method for activating nucleic acid (NA) for a polymerase reaction comprises (a) warming NA to 50-90[deg]C; (b) cooling to a temperature at which no significant reduction in activity of a polymerase occurs and (c) starting reaction by adding a heat-labile polymerase (HLP). An independent claim is included for a method for activating NA for a polymerase reaction comprising warming NA, together with an heat-stable polymerase, to 50-90[deg]C.