TIRF-type microscope optical arrangement, has illumination light carried in beam paths outside microscope objective

摘要

An optical arrangement for microscopic observation has on one side an immersion medium (8) and on the other - a cover glass (3) with florescence excited illumination light passed through the immersion medium and the cover glass onto the boundary/threshold surface (9). The illumination light (4) is carried in beam paths outside the microscope objective (1) on the basis on the diffraction produces by the diffraction grid (6) at least a part (B) of the illumination light is directed at a given angle (delta) on to the boundary surface (9) leading to total reflection at this surface.