Use of thiol-reactive reagents for detecting enzyme activity

摘要

Disclosed are assays useful for detecting enzyme activity. The assays may utilize thiol-reactive reagents. The assays may include reacting a reacting mixture that contains an ATP analog (e.g., ATPbetaS or GTPbetaS) to form an ADP analog (e.g., ATPbetaS or GTPbetaS).