| 摘要 |
The invention relates to a method of preparing DNA fragments by selective fragmentation of nucleic acid fragments. The inventive method comprises a first selection step involving selection of short fragments, consisting in: a) preparing first double-stranded DNA fragments F1 using at least one restriction enzyme E1 which can randomly fragment the nucleic acid sample to be analysed, by generating said DNA fragments F1 with blunt or cohesive ends; b) ligating the ends of the DNA fragments F1 obtained in step (a) to at least one adapter AA'; c) cleaving the DNA fragments F1 obtained in step (b) using a restriction enzyme E2, such as to select a fraction of short fragments F2; and d) using any suitable means to purify the aforementioned fraction of short fragments F2. The inventive method also comprises the following optional step involving the second selection of one or more fragment sub-groups from the fraction of short fragments F2 obtained in step (d), said optional step consisting in: e) ligating the free end of the short fragments F2 obtained in step d) to at least one second complementary adapter BB' (production of fragments F2); and f) amplifying short fragments F2. The invention also relates to the applications of the above-mentioned method for the analysis of genomes and transcriptomes.
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