METHOD FOR DETECTION OF GENE POLYMORPHISM AND METHOD FOR SCREENING OF SUBSTANCE

摘要

It is intended to provide a method for finding a gene polymorphism capable of largely affecting the function of a drug transporter (particularly, ABCB1 or ABCG2) from gene polymorphisms each having any amino acid mutation to clinically determine the influence of the gene polymorphism on a pharmaceutical preparation, or to clinically determine of the gene polymorphism. Disclosed are a method for detection of a gene polymorphism, comprising detecting the presence or absence of a drug transporter gene polymorphism which has an amino acid mutation having any influence on pharmacokinetics in a DNA sample from a subject and predicting the influence of the gene polymorphism on the kinetics of a drug in the subject; and a method for screening of a substance capable of being affected by a drug transporter gene polymorphism which has an amino acid mutation having any influence on pharmacokinetics, the method comprising contacting the drug transporter gene polymorphism with a substance of interest and detecting the occurrence of an interaction between the drug transporter gene polymorphism and the substance. The gene polymorphism is preferably Ala893Pro in ABCB1, or Phe208Ser, Ser248Pro, Phe431Lue or Phe489Leu in ABCG2.