METHOD FOR THE ISOLATION OF CHONDROPROGENITOR CELLS FROM PERIOSTEUM

摘要

A method for isolating chondroprogenitor cell from periosteum is provided to be able to obtain periosteum-derived chondroprogenitor cells using mesenchymal stem cell surface immune indicator and flow cytometry, which is successfully differentiated into cartilage cell used as a cartilage cell therapeutic agent. The method comprises the steps of: (a) after cutting periosteal tissue derived from mammals into fragments, culturing them to isolate cells from the periosteal tissue; (b) and isolating only cells which are positive to mesenchymal stem cell surface marker such as CD9, CD90 and CD116 and negative to hematopoietic stem cell surface marker such as CD45 from the cells isolated by the step(a). To differentiate the periosteum-derived chondroprogenitor cells into cartilage cells, the chondroprogenitor cells are pellet-cultured in a culture medium including TGF-beta3.