Electrotransfer of nucleic acid into tissue cells

摘要

The electrotransfer of a nucleic acid into muscle cells is carried out by an electric stimulation of the tissue as follows:
- first with at least one, preferably a single, pulse of a High Voltage field strength of between 200 and 2000 volts/cm
- second with a single pulse of Low Voltage field strength of between 50 and 140 volts/cm and of duration of between 300 ms and 2000 ms.