METHOD OF CONSTRUCTING MODIFIED PROTEIN

摘要

DNA derived from a microorganism is prepared from a sample containing the microorganism, degenerate primers are prepared based on an amino acid sequence conserved among known proteins nos. 1 through N all having the same type of function, PCR is performed using the degenerate primers in the presence of the DNA derived from the microorganism, the nucleotide sequence of DNA fragment amplified by the PCR is determined and the amino acid sequence coded for by the DNA fragment is also determined, homology between the amino acid sequence coded for by the DNA fragment and amino acid sequences of known proteins is evaluated, a known protein is discovered which has an amino acid sequence having homology with the amino acid sequence coded for by the DNA fragment, the region of the DNA coding for the discovered known protein which codes for the amino acid sequence having the homology is replaced by the DNA fragment to produce a modified DNA, and the modified DNA is expressed to produce a modified protein, thereby achieving high-throughput and efficient functional analysis and screening of a modified protein.