发明名称 |
GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID |
摘要 |
The invention provides f lagellin-based fusion proteins (FBFP) useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacteria useful for expressing the recombinant FBFP on their surfaces. Also included in the invention are genetic constructs useful for making FBFP and methods of using the FBFP. |
申请公布号 |
WO2006072845(A3) |
申请公布日期 |
2007.03.01 |
申请号 |
WO2005IB54022 |
申请日期 |
2005.12.02 |
申请人 |
CSIR;BERGER, ELDIE;LOUW, MAUREEN, ELIZABETH;CRAMPTON, MICHAEL, CRAIG |
发明人 |
BERGER, ELDIE;LOUW, MAUREEN, ELIZABETH;CRAMPTON, MICHAEL, CRAIG |
分类号 |
C07K14/31;C02F1/28;C02F3/34;C07K19/00 |
主分类号 |
C07K14/31 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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