METHODS FOR PROTEOMIC PROFILING USING NON-NATURAL AMINO ACIDS

摘要

The invention provides methods, reagents and systems for incorporating non­natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a "handle" to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues / cells, etc.