Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
摘要
<p>The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.</p>
申请公布号
EP1736554(A3)
申请公布日期
2007.02.21
申请号
EP20060011667
申请日期
1997.05.27
申请人
CORNELL RESEARCH FOUNDATION, INC.;BELGRADER, PHILLIP
发明人
BELGRADER, PHILLIP;BARANY, FRANCIS;LUBIN, MATTHEW;BARANY, GEORGE;HAMMER, ROBERT P.