CORYNEBACTERIUM AMMONIAGENES CJXCV19 KCCM-10692P HAVING LOW ACTIVITY OF DEGRADATION OF GUANINE 5'- MONOPHOSPHATE AND HIGH-THROUGHPUT SCREENING METHOD FOR MUTANT

摘要

A microorganism Corynebacterium ammoniagenes CJXCV19(KCCM-10692P) having low activity of degradation of guanine 5'-monophosphate(GMP) and a high-throughput screening method of the same microorganism are provided to reduce screening time, and produce guanine 5'-monophosphate(GMP) with higher yield from XMP(5'-xanthylic acid). The high-throughput screening method of a mutant of Corynebacterium ammoniagenes CJFXT0301(KCCM-10530) comprises the steps of: culturing the mutants of Corynebacterium ammoniagenes CJFXT0301(KCCM-10530) in a mini-well; inducing conversion reaction of the cultured mutants of Corynebacterium ammoniagenes CJFXT0301(KCCM-10530) mutant into guanine monophosphate(GMP); measuring the concentration of guanine monophosphate(GMP) in the conversion reaction and selecting the mutant showing absorbance of 0.8 at 290 nm when a parent strain shows absorbance of 0.8 or more at 290 nm, wherein the mutant of Corynebacterium ammoniagenes CJFXT0301(KCCM-10530) is Corynebacterium ammoniagenes CJXCV19(KCCM-10692P) which produces XMP(5'-xanthylic acid) and converts it into guanine 5'-monophosphate(GMP) with higher conversion rate.