| 摘要 |
The present invention provides a biosensor that can prevent a mediator from being affected by oxygen, thereby allowing an analyte in a sample solution to be measured rapidly and easily with high accuracy. The biosensor can be produced by providing a substrate having electrodes, applying a solvent containing a mediator, a surfactant, a buffer, and a layered inorganic compound to surfaces of the electrodes to form an inorganic gel layer for preventing natural oxidation of the mediator, and forming an enzyme reagent layer containing an oxidoreductase on the inorganic gel layer. In this biosensor, due to the inorganic gel layer, the mediator having been reduced by the reaction between an analyte and the oxidoreductase can be measured electrochemically, without being reoxidized by dissolved oxygen or the like.
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