SEPARATION OF PROTEINS BASED ON ISOELECTRIC POINT USING SOLID-PHASE BUFFERS

摘要

Proteins can be separated from mixtures, based on their pI values, through the use of a series of chromatographic materials, each comprising a solid buffer and an ion exchange resin. Each solid buffer generates a stable pH, such that passing proteins possess a net charge and can be separated by means of an appropriate ion exchanger. In this fashion, proteins from complex biological fluids can be separated for identification and study.