发明名称 |
ISOLATION OF CPG ISLANDS BY THERMAL SEGREGATION AND ENZYMATIC SELECTION-AMPLIFICATION METHOD |
摘要 |
<p>The present invention concerns isolation, library preparation and selective amplification from a compositionally heterogeneous pool of DNA fragments of a fraction of molecules, such as those originating from promoter CpG islands and characterized by a high GC content. In particular, the process utilizes a heat-induced segregation of DNA molecules into GC-poor, single-stranded molecule fractions and GC-rich, double-stranded molecule fractions, with subsequent enzymatic conversion of the GC-rich, double-stranded DNA molecules into a library, and, optionally, amplification. In specific embodiments, the isolation process is used to generate promoter-enriched genomic and methylome libraries for research and diagnostic applications, for example.</p> |
申请公布号 |
WO2007018602(A1) |
申请公布日期 |
2007.02.15 |
申请号 |
WO2006US07486 |
申请日期 |
2006.03.02 |
申请人 |
RUBICON GENOMICS, INC.;MAKAROV, VLADIMIR, L.;KAMBEROV, EMMANUEL;TARRIER, BRENDAN, J. |
发明人 |
MAKAROV, VLADIMIR, L.;KAMBEROV, EMMANUEL;TARRIER, BRENDAN, J. |
分类号 |
C12Q1/68;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|