METHOD FOR SCREENING FOR PROTEASES AND THEIR SUBSTRATES

摘要

The invention relates to methods for screening for proteases and substrates susceptible to cleavage by proteases. The methods of the invention allow for the screening of proteases in an environment where the proteases are normally present, in particular within a living organism. By inhibiting the activity of at least one protease, preferably at least one first-line protease for a given substrate, which usually produces the majority of proteolytic fragments derived from said substrate and thus, would normally (without inhibiting said activity) mask the activity of a second-line protease, it is possible to identify proteases that perform second-line proteolysis and their recognition sequences.